Although culture remains the gold standard for bacterial detection and identification, its sensitivity in detecting bacterial contamination of blood products is affected by numerous factors including the growth characteristics of the contaminating organism, timing of specimen procurement, specimen volume, and degree of initial bacterial contamination. Numerous studies of culture-based detection systems have been performed. These have demonstrated that the timing of specimen collection is critically important to test sensitivity: cultures obtained on the day of collection invariably fail to demonstrate bacterially contaminated units that could develop clinically significant overgrowth during storage. Because of the small sample volume normally obtained, reliable detection requires time for the organisms to proliferate to levels at which sampling would include adequate numbers of the organism. Currently, most detection system manufacturers recommend at least a 24-hour incubation period prior to sampling.
Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Amikacin Antimicrobial Susceptibility discs.
Ease-of-use is provided by shatterproof spring-loaded cartridges, easy-to-read labeling and end-of-cartridge indicators.
Disc diffusion not only categorizes resistant, intermediate and sensitive organisms through quantitative results but also provides a visual indication of the following:
- Inoculum level
- Presence of contamination
- Resistant mutants
- Beta-lactamase activity
- Antagonism and synergy between antibiotics
With the Antibiotics Disc Dispenser, Antibiotic Discs can be applied simultaneously to a culture medium at even intervals. The antibiotic disk dispenser is suitable for Petri dishes with a diameter of 90 mm.
The base ring of the dispenser can be easily adjusted to the height of the culture medium without additional tools.
The one-handed operation of the dispenser allows rapid application of antibiotic sensitivity tests according to the plate diffusion method and thus saves a lot of time, especially if many samples are to be tested.
Directions for use
The antibiotic disc tubes are inserted into the slots of the dispenser in the desired combination until a click is heard. The loaded dispenser is placed over the Petri dish and a single antibiotic disc per tube is pressed onto the culture medium by gently pressing the plunger with one hand to the end point.
the synthesis of hydrophobic can be improved, aggregated sequences that contain glycine with the use of DMB Dipeptide Building Blocks.
They work in exactly the same way as pseudoproline dipeptides by exploiting the natural propensity of N-alkyl amino acids to disrupt the formation of the secondary structures during peptide assembly.
Their use can result in better and more predictable acylation and deprotection kinetics, enhanced reaction rates, and improved yields, purities and solubility’s of crude products. They can also increase peptide cyclization efficiency.
DMB dipeptides are fully compatible with standard coupling methods used with reagents such as PyBOP, HBTU, DIC/HOBt or HATU.
Ref: Solid-Phase Peptide Synthesis: An Introduction.Knud J. Jensen