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WesternBright Quantum HRP substrate
Quantitative chemiluminescent Western blotting
WesternBright Quantum is formulated to take full advantage of the linear dynamic range of CCD imagers. It provides high sensitivity to detect low abundance proteins, and does not exhibit substrate depletion with high abundance proteins, so has a larger linear dynamic range than other commercially available substrates. A signal linear with respect to protein concentration over 3 orders of magnitude provides the ability to accurately quantify and compare protein amounts in your samples.
Features
- Sensitive – detect attomoles of protein per band.
- Quantitative – linear range of signal with respect to protein amount exceeds 3 orders of magnitude
- Low background – for high signal to noise
- Long lasting signal – image blots hours after substrate incubation
- Versatile – Optimized for CCD imaging, and compatible with film detection. In addition, chemifluorescent emissions can be detected with fluorescence imaging systems
WesternBright Quantum chemiluminescent substrate sets the bar for both sensitivity and quantitative ability. Specially developed for CCD imaging, WesternBright Quantum produces a strong, long-lasting signal with extremely low background, perfect for detecting low abundance proteins. Since it does not exhibit substrate depletion at high protein loads, WesternBright Quantum provides the largest dynamic range of any chemiluminescent substrate for the most quantitative chemiluminescent Western experiments.
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Figure 1. WesternBright Quantum provides a larger linear dynamic range than Amersham ECL or ECL Plus (GE Healthcare) or SuperSignal West Dura (Thermo Scientific). Identical Western blots containing serial dilutions of transferring were probed with a rabbit-anti-transferrin primary antibody and a goat-anti-rabbit secondary antibody conjugated to horseradish peroxidase. The blots were incubated with chemiluminescent substrates as recommended by each manufacturer. All blots were imaged simultaneously for 2 minutes on a CCD imager and display parameters are identical across all images shown. Band intensities were plotted and a best fit linear regression conducted for each substrate. WesternBright Quantum shows the largest linear dynamic range out of all four substrates with the highest R2 value. Bands that fall on the linear part of the curve for each substrate are indicated in the image.
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