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  Top » Catalog » AG-1138-500 grams

TRYPTONE SOY AGAR ISO 9308-1

 [AG-1138-500 grams]
 45.73 

Description

 

For the detection and enumeration of E.coli in water 

FORMULA IN g/l

Casein Triptic Digest

15.00

Sodium chloride

5.00

Soy Peptone

5.00

Bacteriological Agar

15.00

Final pH 7.2 ± 0.1 at 25ºC

 

 

PREPARATION

Suspend 40 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC, mix well and dispense into plates. The prepared medium should be stored at 8-15°C. The color is amber, slightly opalescent.

The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes, discard the medium.

USES

TRYPTONE SOY AGAR is used for the quick and standard test for the detection and count of E. coli and other coliforms by the membrane - filter technique as directed by the ISO 9308-1. 

It is also a general purpose solid medium, particularly useful for the isolation, sensitivity testing and determination of hemolysis with fastidious microorganisms as it does not contain any sugars. Another use of the Tryptone Soy Agar is for the oxidase test according to the traditional method as described in ISO 9308-1. 

The two different peptones it contains allow the cultivation of a great variety of aerobic and facultative bacteria and also such organisms as streptococci, pneumococci and members of the genera Neisseria, Brucella, Corynebacteria, Listeria, Pasteurella and Vibrio

Casein and Soy peptone Casein provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride maintains the osmotic balance and Bacteriological agar is the solidifying agent. 

According to ISO 9308-1, the filtration membrane is placed in the Tryptone Soy Agar and incubated at 36 ± 2°C during 4 - 5 hours. The membrane is then transferred to Tryptone Bile Salts Agar (Cat. 1013) and incubated at 44 ± 0.5°C during 19-20 hours. 

If desired, both media can be combinated into one double-layer Petri dish. In this case, it is advisable to place the membrane on a plate freshly prepared with a double layer consisting of Tryptone Soy Agar and TBA and incubated at 36 ± 2 º C during 4-5 hours and then incubated at 44 ± 0.5 º C for 19-20 hours. 

All indole positive strains which give red colonies when stained with indole reagent are considered as E. coli

For the preparation of blood-plates for hemolysis studies, add 5 - 10% sterile defibrinated blood to sterilized and cooled to 45°C medium.

MICROBIOLOGICAL TEST

The following results were obtained in the performance of the medium from type cultures after incubation at a temperature of 36 ± 2°C and observed after 18 - 24 hours.

Microorganisms

Growth

Escherichia coli ATCC 25922

Good

Klebsiella pneumoniae ATCC 13833

Good

 

BIBLIOGRAPHY

ISO 9308-1 Water quality. Detection and enumeration of Escherichia coli and coliform bacteria.  Part 1 Membrane filtration method

Regulation water quality- Detection and count of Escherichia coli and coliform bacteria. Anon. 1987 J. Food Microbiol., 5: 291 -296.

 

STORAGE

Once opened keep powdered medium closed to avoid hydration.

 

Mfg Info

Manufacturer Info
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