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RapidClean
The fast and easy way to remove protein from RNA and DNA samples
In less than 5 minutes, completely remove protein from aqueous solutions of single- and double-stranded nucleic acids.
Features
- Complete removal of proteins from aqueous solutions of nucleic acids
- Protocol less than 5 minutes, start to finish
- Non-toxic alternative to phenol-chloroform extraction
- Nucleic acid recovery rates >95%
- Recovery of nucleic acids from 5 nucleotides to greater than 40 kb
- Suitable for DNA, RNA, cDNA, microRNA, oligonucleotides
RapidClean is a novel affinity resin designed to remove all protein from aqueous solutions of nucleic acids, providing an alternative to hazardous and lengthy phenol-chloroform procedures. The RapidClean resin binds and removes protein in a 5-minute protocol that combines convenience, speed, and nucleic acid recovery rates in excess of 95%.
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Figure 1. RapidClean completely removes T4 DNA ligase.No detectable ligase activity remains in a reaction mix after exraction with RapidClean (lane 2). EcoRI digested λ-DNA (lane C, Control) was incubated for 16 hours at room temperature with T4 DNA ligase (lane 1) or with the same mixture extracted with RapidClean (lane 2).
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| Figure 2. Extraction with RapidClean completely removes Exonuclease III. ϕX174/HaeIII DNA (Lane M) was digested by a 40 min incubation at 37 °C with 10 units of Exonuclease III (lane 1), but no digestion occured when the enzyme mix was extracted twice with RapidClean before addition of the ϕX174/HaeIII DNA target (lane 2).
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