Mega Base Rapid Agarose for Electrophoresis (AG-D5)

D5 Agarose is a linear polymer with a very high molecular weight, giving gel structures unlike those of traditional agaroses. This characteristic, added to the very low sulfate content, produces an strong intercatenary interaction, yielding a gel with very high gel strength and higher exclusion limit.

Features

• Extremely high gel strength allowing for lower gel concentrations (0.3%), enabling it to be used not only with high molecular weight nucleic acids, including chromosomes, but also with large sized particles like viruses and ribosomes.
• High electrophoretic mobility. DNA mobility is greater when compared with D-1LE. Electrophoresis times are reduced depending upon buffer and agarose concentration used.
• Easy preparation of the gel by simple dissolution in aqueous buffers either by standard boiling or microwaving.
• Greater thermal stability due to high hysteresis (difference between gelling and melting temperatures).
• Exceptionally low absorption of staining agents.
• Absence of toxicity (the alternative is polyacrylamide which can be toxic).

 Applications

• Conventional Electrophoresis: can be used in a wide range of concentrations.
• Pulsed Field Gel Electrophoresis: because of its higher exclusion limit, larger molecules can be separated.
• Blotting.
• Agarose Beads preparation.
• Cell and enzyme immobilization.