These are derivatized by organic synthesis which generates methoxylate groups from the basic agarose structure. The main properties of these agaroses are their low melting and gelling temperatures when compared with standard agaroses. The low melting temperature allows for the recovery of undamaged nucleic acids at temperature lower that its denaturing temperature. The low gelling temperature assures the agarose will be in a liquid state at a temperature range where In-Gel manipulations can be performed without prior extraction of the DNA from the gel slice.
We have two major types of low melting agarose. See below for details:
Hi-RES Low Melting (LM) Agaroses are derivatized by organic synthesis which generates methoxylate groups from the basic agarose structure. The main properties of these agaroses are their low melting and gelling temperatures when compared with standard agaroses.
Hi-RES LM Genetically Quality Tested(GQT) Agarose is a low melting temperature agarose with the highest resolving capacity for large DNA fragments, =1000 bp, including PCR products.
Low Melt Clone, LMSIEVE Agarose is a low melting temperature agarose with the highest resolving capacity for DNA fragments smaller than 1000 bp, especially PCR products ranging from 200 to 800 bp.
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