Transfection

Transfection Reagents From Altogen

Altogen Biosystems is a life sciences company dedicated to the development, marketing and manufacture of cell line specific transfection reagents for intracellular delivery of biomolecules. Efficient delivery of siRNA and plasmid DNA enabled into broad range of cell types and primary cells by advanced formulation of reagents and peculiar design of protocols. Altogen Biosystems develops polymer-, lipid-, and dendrimer-based transfection technology by applying expertise in molecular biology, combinatorial chemistry, and cell biology.

Altogen Custom Services offer stable cell line generation and RNA Interference (RNAi) gene silencing services. Our cell culture scientists can transform your cell line to stably express vector or gene of interest. RNAi service include design and synthesis of siRNA targeting gene of interest, development of efficient transfection reagent, and optimization of siRNA transfection conditions to enable highly efficient gene silencing in your cell line or cultured primary cells.

Transfection is the transient or stable introduction of exogenous molecules and genetic material, RNA or DNA, into cultured cells and is commonly utilized in biological laboratories for studying gene function, modulation of gene expression, biochemical mapping, mutational analysis, and protein production. Scientists use various carrier molecules to enable gene delivery of such molecules as plasmid DNA (pDNA), messenger RNA (mRNA), short interfering RNA (siRNA), and microRNA (miRNA) into cancer cell lines and primary cells. Unfortunately, no single delivery method or transfection reagent can be applied to all types of cells; cellular cytotoxicity and transfection efficiencies vary dramatically depending on the reagent, protocol and cell type being utilized. Altogen Biosystems offers pre-optimized transfection kits for more than 60 cell types.

Altogen Biosystems presents featured cell line-specific transfection kits: Fibroblast, HepG2, A549, MDA-MB, HEK-293, Astrocytes, CHO, COS-7, HUVEC, LNCaP. Each kit contains cell type specific Transfection Reagent, Transfection Enhancer Reagent, and recommended transfection protocol. Lipid-based and polymer-based ALTOGEN® In Vivo Transfection Reagents enable delivery of functional RNA and DNA molecules in vivo.

Read recent article by Dr.Lakshmanan "A549 Transfection Reagent From Altogen": Link

Visit Altogen's Transfection Resource: RNAi applications, stable and transient transfection, delivery methods, electroporation, microinjection, dendrimer-based, non-viral and virus-mediated gene delivery, liposome and non-liposomal reagents.

Review by Cold Spring Harbor Laboratory Press Scientific Supplies Portal: Link