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Markers & Plasmids
| The following plasmid's can be obtained from biotools see product listing s for details |
| Plasmid pKT1 is a pUC-derived plasmid containing the kat cassette. This cassette is expressed under the control of a promoter active in Escherichia coli and Thermus thermophilus. The cassette codifies for kanamycine resistance (30 mg / ml). The cassette can be easily extracted by using the restriction sites included in its flanking polylinker.(Lasa et al (1992). Mol.Microbiol., 11: 1555-1564). Patent number P9301522.
Plasmid pMK18 (1 µg a 100 ng / µl) - Cat. No. 31.018Plasmid pMK18 has been obtained from Escherichia coli and Thermus thermophilus sequences, contaning ori sites. Therefore, it is a shuttle vector, that can be used for thermostabilisation studies involving transformation of both organisms. The plasmid contains the kat cassette (see pKT1) as a selectable marker.De Grado, M. Ph.D. Thesis, UAM, Madrid, Spain). Patent number P9301522.Plasmid pMKE1 (1 µg a 150 ng / µl) - Cat. No. 31.119
Plasmid pMKE1 is a pUC-derived plasmid containing the kat cassette. The cassette codifies for kanamycine resistance (30 mg / ml). The cassette can be easily extracted by using the restriction sites included in its flanking polylinker. Also, the plasmid includes the Pnar promoter, for nitrate-induced expression.
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| Also the following ladders are available
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