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  Top » Catalog » K-02101-025

Afyon SDS-PAGE Sample Preparation Kit

 [K-02101-025]

Description

Afyon SDS-PAGE Sample Preparation Kit

 

Afyon SDS-PAGE Sample Preparation Kit

Fast, easy concentration and purification of samples for SDS-PAGE

Afyon SDS-PAGE sample preparation kit is a fast, efficient way to concentrate protein samples and remove buffer components that may interfere with electrophoresis. In less than 10 minutes, samples are ready for SDS-PAGE or immunoblotting. The Afyon protocol is easily scaled up and can be used for routine preparation of samples for electrophoresis.

 
Features
  • Quickly remove contaminants that can interfere with electrophoresis (GuHCl, urea, Ammonium sulfate, etc)
  • Protein samples ready to load in less than 10 minutes
  • Safe and non-toxic - no DMSO required
  • Many times faster than concentration and buffer exchange via ultrafiltration or dialysis
  • Compatible with SDS-PAGE and downstream Western blotting

The Afyon SDS-PAGE sample preparation kit provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis. The fast, efficient protocol generates samples ready to load on a gel in less than ten minutes, much more quickly than can be achieved with alternate methods such as dialysis, or acetone or TCA precipitation. Additionally, the Afyon protocol is easily scaled up, allowing multiple samples to be prepared in parallel.

Afyon Figure 1

Afyon concentration is compatible with Western blot detection. Western blots were created with samples of A431 cell extract (panels a and b, lane 2), diluted cell extract (lane 3) and the diluted extract concentrated using the Afyon protocol (lane 4). The blots were detected using the WesternBright MCF multicolor fluorescent Western blotting kit. No bands were detectable in the diluted cell extract when stained for either GAPDH (panel a, lane 3) or SRC (panel b, lane 3). However, after Afyon concentration, bands are easily visualized for both proteins (lanes 4). Band intensities are indicated.

 

 
Afyon SDS-PAGE Sample Preparation Kit | K-02101-010 rxn
 £90.63 

Qty:

 
Afyon SDS-PAGE Sample Preparation kit | K-02101-025 rxn
 £184.38 

Qty:

 
Non-reducing protein sample loading buffer (2X) | R-03018-B10 1ml
 £8.75 

Qty:

More Info

Product Data Sheet:  Data Sheet
Download MSDS:  MSDS;
Application Notes:  Application Note
Instruction Leaflets:  User Manual
Procedural Guides:  Protein analysis guide

Mfg Info

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Extra Info
This product was added to our catalog on Friday 02 December, 2011.Notify me of updates to Afyon SDS-PAGE Sample Preparation Kit
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